How to sequence and assemble a large eukaryote genome with long reads in 2015 [Link] →

If you have any interest in the latest methods of DNA sequencing and/or genome assembly, you really owe it to yourself to be following Lex Nederbragt's excellent In between lines of code blog. Today's post offers some useful advice:

Main advice: bite the bullet and get the budget to get 100x coverage in long PacBio reads. 50-60x is really the minimum.